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Protein Self-Assembly pp 117–129Cite as

Differential Scanning Calorimetry to Quantify Heat-Induced Aggregation in Concentrated Protein Solutions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2039))

Abstract

Differential scanning calorimetry (DSC) is an important technique to measure the thermodynamics of protein unfolding (or folding). Information including the temperature for the onset of unfolding, the melt transition temperature (Tm), enthalpy of unfolding (ΔH), and refolding index (RI) are useful for evaluating the heat stability of proteins for a range of biochemical, structural biology, industrial, and pharmaceutical applications. We describe a procedure for careful sample preparation of proteins for DSC measurements and data analysis to determine a range of thermodynamic parameters. In particular, we highlight a measure of protein refolding following complete thermal denaturation (RI), which quantifies the proportion of protein lost to irreversible aggregation after thermal denaturation.

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Acknowledgments

The research work presented in this chapter was made possible through the financial support of Enterprise Ireland under grant number [IP/2015 0358] and the Synthesis and Solid State Pharmaceutical Centre, cofunded under the European Regional Development Fund, and the Department of Agriculture Food and Marine, FIRM grant number [11/F/037], SFI Stokes Lectureship to J.J.M., and Maynooth University Teaching Fellowship to A.B.

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Authors and Affiliations

  1. Department of Chemistry, Maynooth University, Maynooth, Kildare, Ireland

    Matthew R. Jacobs, Mark Grace & Alice Blumlein

  2. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland

    Jennifer J. McManus

Authors
  1. Matthew R. Jacobs
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  2. Mark Grace
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  3. Alice Blumlein
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Correspondence to Jennifer J. McManus .

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  1. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland

    Jennifer J. McManus

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Jacobs, M.R., Grace, M., Blumlein, A., McManus, J.J. (2019). Differential Scanning Calorimetry to Quantify Heat-Induced Aggregation in Concentrated Protein Solutions. In: McManus, J. (eds) Protein Self-Assembly. Methods in Molecular Biology, vol 2039. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9678-0_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9678-0_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9677-3

  • Online ISBN: 978-1-4939-9678-0

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