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Measuring Protein Solubility

  • Neer AsherieEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2039)

Abstract

Protein solubility determines the conditions under which the protein will remain in solution. As a result, it is an important quantity in applications that involve concentrated protein solutions. Here I describe the solubility measurement of the protein thaumatin in the presence of tartrate ions as a function of temperature. This method can be used to measure the solubility of other proteins.

Key words

Protein Solubility Thaumatin Protein crystallization Phase diagrams 

Notes

Acknowledgments

I gratefully acknowledge financial support from the National Science Foundation (DMR 0901260 and 1206416) and Yeshiva University.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of PhysicsYeshiva UniversityNew YorkUSA
  2. 2.Department of BiologyYeshiva UniversityNew YorkUSA

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