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Light Scattering to Quantify Protein–Protein Interactions at High Protein Concentrations

  • Mahlet A. Woldeyes
  • Cesar Calero-Rubio
  • Eric M. Furst
  • Christopher J. RobertsEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2039)

Abstract

Static and dynamic (laser) light scattering (SLS and DLS, respectively) can be used to measure the so-called weak or colloidal protein–protein interactions in solution from low to high protein concentrations (c2). This chapter describes a methodology to measure protein–protein self-interactions using SLS and DLS, with illustrative examples for monoclonal antibody solutions from low to high protein concentrations (c2 ~ 1–102 g/L).

Key words

Protein–protein interactions Static light scattering Dynamic light scattering Structure factor Hydrodynamic factor 

Notes

Acknowledgments

Support from the National Science Foundation (NSF GRF to M.A.W.), Amgen, and the National Institute of Health (NIH R01EB006006) are gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Mahlet A. Woldeyes
    • 1
  • Cesar Calero-Rubio
    • 1
  • Eric M. Furst
    • 1
  • Christopher J. Roberts
    • 1
    Email author
  1. 1.Department of Chemical and Biomolecular EngineeringUniversity of DelawareNewarkUSA

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