Light Scattering to Quantify Protein–Protein Interactions at High Protein Concentrations
Static and dynamic (laser) light scattering (SLS and DLS, respectively) can be used to measure the so-called weak or colloidal protein–protein interactions in solution from low to high protein concentrations (c2). This chapter describes a methodology to measure protein–protein self-interactions using SLS and DLS, with illustrative examples for monoclonal antibody solutions from low to high protein concentrations (c2 ~ 1–102 g/L).
Key wordsProtein–protein interactions Static light scattering Dynamic light scattering Structure factor Hydrodynamic factor
Support from the National Science Foundation (NSF GRF to M.A.W.), Amgen, and the National Institute of Health (NIH R01EB006006) are gratefully acknowledged.
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