Abstract
In recent years, nanoparticle tracking analysis (NTA) has emerged as an alternative tool for particle size characterization. Especially when examining polydisperse systems, individual particle to particle tracking allows for higher peak resolution than dynamic light scattering techniques. However, NTA requires an experienced user with a good insight into how the different settings can affect the determination of particle size and size distributions. This chapter provides a guideline for protein aggregation studies using the example of temperature-induced aggregation of IgG at low concentration.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Malloy A, Carr B (2006) Nanoparticle tracking analysis – the halo™ system. Part Part Syst Charact 23:197–204
Filipe V, Hawe A, Jiskoot W (2010) Critical evaluation of nanoparticle tracking analysis (NTA) by NanoSight for the measurement of nanoparticles and protein aggregates. Pharm Res 27:796–810
Ribeiro LN de M, Couto VM, Fraceto LF et al (2018) Use of nanoparticle concentration as a tool to understand the structural properties of colloids. Sci Rep 8:982
Maas SLN, De Vrij, Van Der Vlist EJ et al (2015) Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics. J Control Release 200:87–96
Gardiner C, Ferreira YJ, Dragovic RA et al (2013) Extracellular vesicle sezing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles 2:19671
Rather GM, Mukherjee J, Halling PJ et al (2012) Activation of alpha chymotrypsin by three phase partitioning is accompanied by aggregation. PLoS One 7:e49241
Sediq AS, Nejadnik MR, El Bialy I et al (2015) Protein–polyelectrolyte interactions: monitoring particle formation and growth by nanoparticle tracking analysis and flow imaging microscopy. Eur J Pharm Biopharm 93:339–345
Krueger AB, Carnell P, Carpenter JF (2016) Characterization of factors affecting nanoparticle tracking analysis results with synthetic and protein nanoparticles. J Pharm Sci 105:1434–1443
Patois E, Capelle MAH, Palais C et al (2012) Evaluation of nanoparticle tracking analysis (NTA) in the characterization of therapeutic antibodies and seasonal influenza vaccines: pros and cons. J Drug Deliv Sci Technol 22:427–433
Acknowledgments
This chapter has emanated from research conducted with the financial support of Enterprise Ireland under Grant Number [IP/2015 0358] and the Synthesis and Solid State Pharmaceutical Centre, funded by Science Foundation Ireland (SFI) under Grant Number [12/RC/2275], cofunded under the European Regional Development Fund.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Sladek, S., McComiskey, K., Healy, A.M., Tajber, L. (2019). Nanoparticle Tracking Analysis to Examine the Temperature-Induced Aggregation of Proteins. In: McManus, J. (eds) Protein Self-Assembly. Methods in Molecular Biology, vol 2039. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9678-0_10
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9678-0_10
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9677-3
Online ISBN: 978-1-4939-9678-0
eBook Packages: Springer Protocols