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Measuring Nonspecific Protein–Protein Interactions by Dynamic Light Scattering

  • Daniel Corbett
  • Jordan W. Bye
  • Robin A. CurtisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2039)

Abstract

Dynamic light scattering has become a method of choice for measuring and quantifying weak, nonspecific protein–protein interactions due to its ease of use, minimal sample consumption, and amenability to high-throughput screening via plate readers. A procedure is given on how to prepare protein samples, carry out measurements by commonly used experimental setups including flow through systems, plate readers, and cuvettes, and analyze the correlation functions to obtain diffusion coefficient data. The chapter concludes by a theoretical section that derives and rationalizes the correlation between diffusion coefficient measurements and protein–protein interactions.

Key words

Biopharmaceuticals Second virial coefficients Protein crystallization Protein aggregation Osmometry 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Daniel Corbett
    • 1
  • Jordan W. Bye
    • 1
  • Robin A. Curtis
    • 1
    Email author
  1. 1.School of Chemical Engineering and Analytical ScienceThe University of ManchesterManchesterUK

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