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Assessing Oligonucleotide Binding to Double-Stranded DNA

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Oligonucleotide-Based Therapies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2036))

Abstract

Sequence-specific targeting of double-stranded DNA (dsDNA) using synthetic oligonucleotides (ONs) has been under investigation in different therapeutic approaches. Several methods can be used to evaluate ONs effect and binding capacity to their target sequence. Here we describe some of the methods, which have been frequently used for assessing ONs binding to dsDNA.

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Acknowledgments

This work was supported by the European Union’s Horizon 2020 under the Marie Skłodowska-Curie grant agreement No 721613, the Swedish Research Council, the Stockholm County Council, Hjärnfonden, and Vinnova/SweLife.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Center for Advanced Therapies, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden

    Negin Mozafari & Tea Umek

Authors
  1. Negin Mozafari
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  2. Tea Umek
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Corresponding authors

Correspondence to Negin Mozafari or Tea Umek .

Editor information

Editors and Affiliations

  1. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Olof Gissberg

  2. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Rula Zain

  3. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Karin E. Lundin

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Mozafari, N., Umek, T. (2019). Assessing Oligonucleotide Binding to Double-Stranded DNA. In: Gissberg, O., Zain, R., Lundin, K. (eds) Oligonucleotide-Based Therapies. Methods in Molecular Biology, vol 2036. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9670-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9670-4_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9669-8

  • Online ISBN: 978-1-4939-9670-4

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