Studying the Biotransformation of Phosphorothioate-Containing Oligonucleotide Drugs by LC-MS
Across the pharmaceutical industry, there is increasing interest and need to investigate the biotransformation of oligonucleotide drugs. The method of choice is high-resolution mass spectrometry due to its unmet sensitivity and specificity.
Here, we describe a method developed and applied in our laboratory studying the biotransformation of phosphorothioate-containing oligonucleotide drugs. This method is based on capillary flow liquid chromatography with column switching coupled to high-resolution mass spectrometry.
Key wordsOligonucleotide biotransformation UPLC Column switching High resolution mass spectrometry All ion fragmentation
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