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Analysis of Synaptic Protein–Protein Interaction by Cross-linking Mass Spectrometry

Protocol
Part of the Neuromethods book series (NM, volume 146)

Abstract

Protein–protein interactions and their dynamics define organelles’ structures and drive all aspects of cellular functions. In this chapter I describe a cross-linking mass spectrometry protocol that makes use of a newly developed MS-cleavable cross-linker to identify the protein pairs based on their proximity as defined by the length of the cross-linker. Unlike other approaches, such as immunoprecipitation-based interaction proteomics or yeast-two hybrid analysis, this protocol could characterize protein–protein interactions in a more physiological setting and allows the detection of native protein complexes contained in the mouse synaptosome.

Keywords

Synapse DSSO Cross-linking Mass Spectrometry Protein Protein interaction 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)BerlinGermany

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