Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3)

  • Ka Wan LiEmail author
Part of the Neuromethods book series (NM, volume 146)


The success of a proteomics experiment critically depends on the correct execution of the sample preparation. Gel-based sample preparation is a well-proven method widely used in qualitative and quantitative proteomics. Recently, sample preparation in a single vessel has gained popularity because of the ease to perform, the possibility for large-scale analysis, and the potential to improve sensitivity. In this chapter I describe two sample preparation protocols, filter-aided sample preparation (FASP) and single-pot solid-phase sample preparation (SP3).


Sample preparation FASP SP3 Proteomics 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam NeuroscienceVrije Universiteit AmsterdamAmsterdamThe Netherlands

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