Laser Microdissection for Spatial Proteomics of Postmortem Brain Tissue

  • David C. HondiusEmail author
  • Jeroen J. M. Hoozemans
  • Annemieke J. M. Rozemuller
  • August B. Smit
Part of the Neuromethods book series (NM, volume 146)


Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.


Laser microdissection Laser capture Postmortem tissue Human brain Proteomics Mass spectrometry Immunohistochemistry Single cell 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • David C. Hondius
    • 1
    • 2
    Email author
  • Jeroen J. M. Hoozemans
    • 2
  • Annemieke J. M. Rozemuller
    • 2
  • August B. Smit
    • 3
  1. 1.Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam NeuroscienceVrije Universiteit AmsterdamAmsterdamThe Netherlands
  2. 2.Department of Pathology, Amsterdam NeuroscienceAmsterdam UMCAmsterdamThe Netherlands
  3. 3.Department of Molecular and Cellular Neurobiology, Faculty of Science, Center for Neurogenomics and Cognitive Research, Amsterdam NeuroscienceVrije Universiteit AmsterdamAmsterdamThe Netherlands

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