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Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue

  • Sabine SpijkerEmail author
  • Leonidas Faliagkas
  • Priyanka Rao-Ruiz
Protocol
Part of the Neuromethods book series (NM, volume 146)

Abstract

Previously we reported on a free-hand dissection guide for proteomics samples, where the major focus was on dissection of fresh brain tissue. Although the fresh brain provides clear advantages, such as being able to see structures in very great detail by differences in color and shade, it also has some disadvantages. This particularly becomes evident when processing multiple samples within a short time-window, e.g., after a physiological stimulus. In this chapter, we discuss some of the current methods to take tissue samples from frozen tissue, keeping in mind the requirements for subcellular isolation (e.g., synaptosomes) of proteomics samples. In addition, we provide a step-by-step protocol to improve standardization of tissue dissection in your lab.

Keywords

Guided free-hand dissection Mouse Rat Brain Frozen tissue 

References

  1. 1.
    Spiker S (2011) Dissection of rodent brain regions. In: Li KW (ed) Neuroproteomics. Humana Press, Totowa, NJ, pp 13–26CrossRefGoogle Scholar
  2. 2.
    Paxinos G, Franklin KBJ (2001) The mouse brain in stereotaxic coordinates: hard cover edition. ElsevierGoogle Scholar
  3. 3.
    Paxinos G, Watson C (2006) The rat brain in stereotaxic coordinates: hard cover edition. ElsevierGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sabine Spijker
    • 1
    Email author
  • Leonidas Faliagkas
    • 1
  • Priyanka Rao-Ruiz
    • 1
  1. 1.Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam NeuroscienceVrije Universiteit AmsterdamAmsterdamThe Netherlands

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