A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder

  • Martina Rosato
  • Titia Gebuis
  • Iryna Paliukhovich
  • Sven Stringer
  • Patrick F. Sullivan
  • August B. Smit
  • Ronald E. van KesterenEmail author
Part of the Neuromethods book series (NM, volume 146)


Studying biological mechanisms underlying neuropsychiatric disorders is highly challenging as many risk genes are associated with these disorders. This complexity requires research approaches to reliably dissect the cell biology of the risk genes involved. Here, we describe a combined cellomics–proteomics approach that allows (a) medium-throughput functional screening and unbiased selection of important risk genes, and (b) discovery of common functional pathways and interactome connections of selected risk genes. The overlay of pathway and proteome data from selected genes in a biological context can be used to formulate new testable hypothesis of both the genetics and the biology of the disorders.


Cellomics High-content screening Proteomics Psychiatric disorders 



MR was supported by the European grant U-FP7 MC-ITN IN-SENS (#607616) and the Schizophrenia United Network (SUN) project. We gratefully acknowledge support from the Swedish Research Council (Vetenskapsrådet, award D0886501).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Martina Rosato
    • 1
  • Titia Gebuis
    • 1
  • Iryna Paliukhovich
    • 1
  • Sven Stringer
    • 2
  • Patrick F. Sullivan
    • 3
    • 4
  • August B. Smit
    • 5
  • Ronald E. van Kesteren
    • 1
    Email author
  1. 1.Department of Molecular and Cellular Neurobiology, Faculty of Science, Center for Neurogenomics and Cognitive ResearchVrije Universiteit AmsterdamAmsterdamThe Netherlands
  2. 2.Department of Complex Trait Genetics, Faculty of Science, Center for Neurogenomics and Cognitive ResearchVrije Universiteit AmsterdamAmsterdamThe Netherlands
  3. 3.Department of Medical Epidemiology and BiostatisticsKarolinska InstitutetStockholmSweden
  4. 4.Department of GeneticsUniversity of North CarolinaChapel HillUSA
  5. 5.Department of Molecular and Cellular Neurobiology, Faculty of Science, Center for Neurogenomics and Cognitive Research, Amsterdam NeuroscienceVrije Universiteit AmsterdamAmsterdamThe Netherlands

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