A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder
Studying biological mechanisms underlying neuropsychiatric disorders is highly challenging as many risk genes are associated with these disorders. This complexity requires research approaches to reliably dissect the cell biology of the risk genes involved. Here, we describe a combined cellomics–proteomics approach that allows (a) medium-throughput functional screening and unbiased selection of important risk genes, and (b) discovery of common functional pathways and interactome connections of selected risk genes. The overlay of pathway and proteome data from selected genes in a biological context can be used to formulate new testable hypothesis of both the genetics and the biology of the disorders.
KeywordsCellomics High-content screening Proteomics Psychiatric disorders
MR was supported by the European grant U-FP7 MC-ITN IN-SENS (#607616) and the Schizophrenia United Network (SUN) project. We gratefully acknowledge support from the Swedish Research Council (Vetenskapsrådet, award D0886501).
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