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2D-DIGE as a Tool in Neuroproteomics

  • Florian WeilandEmail author
Protocol
Part of the Neuromethods book series (NM, volume 146)

Abstract

Neuroproteomics encompasses the study of all protein-related dynamics of the nervous system, not only on a morphological level in development and disease but also functional aspects of the process of learning as well as changes in clinical conditions like depression and addiction. Detection of these changes in protein abundance under defined conditions is the field of differential proteome analysis, with two-dimensional difference gel electrophoresis (2D-DIGE) currently being the most comprehensive technique to quantify these changes while retaining information about isoforms and posttranslational modifications. This chapter focusses on technical aspects of 2D-DIGE as well as a brief overview of successful applications of this technique in neuroproteomics.

Keywords

2D-DIGE Proteomics 

Notes

Acknowledgments

The author wants to thank Maithili Shroff for proof-reading the manuscript.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, Sir James Black Centre, School of Life SciencesUniversity of DundeeDundeeUK

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