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Microglia pp 231–241Cite as

Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia

Part of the Methods in Molecular Biology book series (MIMB,volume 2034)


Similar to many nonexcitable cells, microglia utilize intracellular Ca2+ signaling for the communication with each other as well as neurons and astrocytes and for triggering a magnitude of their executive functions. However, in vivo measurements of the intracellular Ca2+ dynamics in microglia have been challenging due to technical reasons. Here, we describe an approach utilizing a single-cell electroporation technique to facilitate the study of microglial Ca2+ signaling in the living brain.

Key words

  • Microglia
  • In vivo calcium imaging
  • Electroporation
  • 2-photon microscopy

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  • DOI: 10.1007/978-1-4939-9658-2_17
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This work was partially supported by the VolkswagenStiftung (grant no. 90233) to O.G.

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Correspondence to Olga Garaschuk .

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Brawek, B., Garaschuk, O. (2019). Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia. In: Garaschuk, O., Verkhratsky, A. (eds) Microglia. Methods in Molecular Biology, vol 2034. Humana, New York, NY.

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  • Print ISBN: 978-1-4939-9657-5

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