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Microglia pp 231–241Cite as

Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia

Part of the Methods in Molecular Biology book series (MIMB,volume 2034)

Abstract

Similar to many nonexcitable cells, microglia utilize intracellular Ca2+ signaling for the communication with each other as well as neurons and astrocytes and for triggering a magnitude of their executive functions. However, in vivo measurements of the intracellular Ca2+ dynamics in microglia have been challenging due to technical reasons. Here, we describe an approach utilizing a single-cell electroporation technique to facilitate the study of microglial Ca2+ signaling in the living brain.

Key words

  • Microglia
  • In vivo calcium imaging
  • Electroporation
  • 2-photon microscopy

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  • DOI: 10.1007/978-1-4939-9658-2_17
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Acknowledgments

This work was partially supported by the VolkswagenStiftung (grant no. 90233) to O.G.

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Correspondence to Olga Garaschuk .

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Brawek, B., Garaschuk, O. (2019). Single-Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia. In: Garaschuk, O., Verkhratsky, A. (eds) Microglia. Methods in Molecular Biology, vol 2034. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9658-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9658-2_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9657-5

  • Online ISBN: 978-1-4939-9658-2

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