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SpyTag–SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo

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Bioconjugation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2033))

Abstract

The emergence of “molecular superglue,” such as SpyTag–SpyCatcher chemistry, has tremendously expanded our capability in manipulating protein shape and architecture via conjugation. Telechelic proteins bearing the SpyTag and SpyCatcher reactive sequences can be expressed and purified for bioconjugation in vitro, giving protein conjugates, branched proteins, and circular proteins. By encoding both reactive sequences in the same construct for expression in vivo, the nascent protein undergoes programmed posttranslational modification guided by protein folding and reaction, leading to diverse nonlinear topologies in situ. In this chapter, we present the SpyTag–SpyCatcher chemistry as a versatile platform for protein bioconjugation and topology engineering.

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Acknowledgments

We thank Prof. Mark Howarth at Oxford University for helpful discussions and suggestions. We are grateful for the financial support from the National Natural Science Foundation of China (Grants 21474003, 91427304), 863 Program (2015AA020941), and “1000 Plan (Youth).”

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Authors and Affiliations

  1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, People’s Republic of China

    Xiao-Wei Wang & Wen-Bin Zhang

Authors
  1. Xiao-Wei Wang
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  2. Wen-Bin Zhang
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Correspondence to Wen-Bin Zhang .

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Editors and Affiliations

  1. Cellular and Molecular Immunology Laboratory, Vrije Universiteit Brussel, Brussels, Belgium

    Sam Massa

  2. In Vivo Cellular and Molecular Imaging Laboratory (ICMI), Vrije Universiteit Brussel, Brussels, Belgium

    Nick Devoogdt

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Wang, XW., Zhang, WB. (2019). SpyTag–SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo. In: Massa, S., Devoogdt, N. (eds) Bioconjugation. Methods in Molecular Biology, vol 2033. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9654-4_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9654-4_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9653-7

  • Online ISBN: 978-1-4939-9654-4

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