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Immunophenotyping of Human B Lymphocytes in Blood and in Adipose Tissue

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Immunophenotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2032))

Abstract

The human obese subcutaneous adipose tissue (SAT) contributes to systemic and B cell intrinsic inflammation, reduced B cell responses, and increased secretion of autoimmune antibodies. Immune cells are recruited to the SAT by chemokines released by both adipocytes and infiltrating immune cells. We describe here the characterization of B lymphocytes from the SAT and blood (control) of obese females undergoing weight reduction surgeries (breast reduction or panniculectomy). We show how to isolate the immune cells from the blood and SAT, how to characterize B cells and their subsets, and how to measure markers of activation and/or transcription factors in SAT-derived B cells and B cell subsets. We also show how to evaluate other immune cell types infiltrating the SAT, including T cells, NK cells, monocyte/macrophages, in order to measure relative proportions of these cell types as compared to the blood.

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Acknowledgments

This study was supported by NIH R56 AG32576 (DF/BBB), R21 AG042826 (DF/BBB), R21 AI096446 (BBB/DF), R56 AG059719 [4].

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, USA

    Alain Diaz, Maria Romero, Daniela Frasca & Bonnie B. Blomberg

  2. Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA

    Bonnie B. Blomberg

Authors
  1. Alain Diaz
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  2. Maria Romero
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  3. Daniela Frasca
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  4. Bonnie B. Blomberg
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Corresponding author

Correspondence to Bonnie B. Blomberg .

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Editors and Affiliations

  1. Frederick, MD, USA

    J. Philip McCoy, Jr

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Diaz, A., Romero, M., Frasca, D., Blomberg, B.B. (2019). Immunophenotyping of Human B Lymphocytes in Blood and in Adipose Tissue. In: McCoy, Jr, J. (eds) Immunophenotyping. Methods in Molecular Biology, vol 2032. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9650-6_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9650-6_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9649-0

  • Online ISBN: 978-1-4939-9650-6

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