Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles

  • Nicholas Wanner
  • Kewal AsosinghEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2032)


Flow-cytometric detection of circulating endothelial cells and endothelial microparticles is an essential tool in studies of vascular diseases. Here we describe the principles and detailed methods for human blood sample processing, storage, labeling, and gating of circulating endothelial elements.

Key words

Endothelial Flow cytometry Microparticle Immunophenotyping 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Inflammation and ImmunityCleveland Clinic, Lerner Research InstituteClevelandUSA

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