Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow-Cytometric Analysis

  • Abdullah Alsuwaidan
  • Franklin Fuda
  • Weina Chen
  • Mingyi ChenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2032)


Dendritic cells (DCs) are powerful antigen presenting cells that are involved in regulating immune response. Plasmacytoid dendritic cells (pDCs) are subtype of DCs that present in small quantity in the bone marrow, peripheral blood, and lymph nodes. They are important component of our immune system in normal condition and diseases. They activate T cells and play a critical role in immune tolerance. In this chapter we review the immunophenotypic features of pDCs and provide a practical protocol for pDCs enumeration in the peripheral blood and bone marrow samples.

Key words

Flow cytometry Dendritic cells Plasmacytoid dendritic cells Immunophenotyping 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Abdullah Alsuwaidan
    • 1
  • Franklin Fuda
    • 2
  • Weina Chen
    • 2
  • Mingyi Chen
    • 2
    Email author
  1. 1.Division of Molecular and Genomic PathologyUniversity of Pittsburgh Medical CenterPittsburghUSA
  2. 2.Department of PathologyUniversity of Texas Southwestern Medical CenterDallasUSA

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