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Detection of UV-Induced Thymine Dimers

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Book cover Genotoxicity Assessment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2031))

Abstract

Ultraviolet rays induce interstrand and intrastrand DNA cross-links, usually thymine–thymine cyclobutane dimer (T–T) and thymine–thymine pyrimidine–pyrimidone (6-4) photoproduct (T (6-4) T). These DNA cross-links, if left unrepaired, increase the risk of these mutation being incorporated in the genetic material (i.e., DNA). Numerous studies have reported the mutagenic potential of above mentioned DNA adducts in prokaryotes, yeast and mammalian cells. Different techniques have been developed to identify such DNA adducts such as immuno-Southern blotting. This is a routinely used quantitative method to determine especially the amount of thymine dimers formed, following irradiation. In this chapter, the detailed methodology to identify thymine dimers formation is provided, using specific antibody against these adducts.

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Acknowledgments

VKY and PA are PhD students under AcSIR and receive doctoral fellowship from Council for Scientific and Industrial Research (CSIR). Research in AK lab is supported by DBT-Wellcome Trust India Alliance, Department of Biotechnology, and SERB (Department of Science and Technology).

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Authors and Affiliations

  1. Genome & Cell Integrity Laboratory, Systems Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research, Lucknow, India

    Vipin Kumar Yadav, Poorwa Awasthi & Amit Kumar

Authors
  1. Vipin Kumar Yadav
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  2. Poorwa Awasthi
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  3. Amit Kumar
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Corresponding author

Correspondence to Amit Kumar .

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Editors and Affiliations

  1. CSIR—Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh, India

    Alok Dhawan

  2. New Delhi, Delhi, India

    Mahima Bajpayee

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Yadav, V.K., Awasthi, P., Kumar, A. (2019). Detection of UV-Induced Thymine Dimers. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 2031. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9646-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9646-9_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9645-2

  • Online ISBN: 978-1-4939-9646-9

  • eBook Packages: Springer Protocols

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