Two-Dimensional Electrophoresis and Mass Spectrometry for Protein Identification
Two-dimensional electrophoresis is useful for separation of individual proteins that can be easily isolated and processed with mass spectrometry for their identification. Here we describe a simplified method of 2D electrophoresis with aim to help beginners in optimization of protein separation protocol, which can be further modified to enhance protein spot resolution. Current protocol can be used in different fields of biology including progenitor cells.
Key words2D Electrophoresis Proteins Proteomics Mass spectrometry Isoelectric focusing SDS-PAGE
We acknowledge Dipavo Banerjee and Divya Khandekar for manuscript preparation. We thank National Centre for Cell Science, Pune, India, for supporting the related original diabetes research.