Two-Dimensional Electrophoresis and Mass Spectrometry for Protein Identification

  • Amaresh K. RanjanEmail author
  • Anil Gulati
Part of the Methods in Molecular Biology book series (MIMB, volume 2029)


Two-dimensional electrophoresis is useful for separation of individual proteins that can be easily isolated and processed with mass spectrometry for their identification. Here we describe a simplified method of 2D electrophoresis with aim to help beginners in optimization of protein separation protocol, which can be further modified to enhance protein spot resolution. Current protocol can be used in different fields of biology including progenitor cells.

Key words

2D Electrophoresis Proteins Proteomics Mass spectrometry Isoelectric focusing SDS-PAGE 



We acknowledge Dipavo Banerjee and Divya Khandekar for manuscript preparation. We thank National Centre for Cell Science, Pune, India, for supporting the related original diabetes research.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Chicago College of PharmacyMidwestern UniversityDowners GroveUSA

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