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Identification and Analysis of Mouse Erythroid Progenitor Cells

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Book cover Progenitor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2029))

Abstract

The most common cell type in the human body, the red blood cell or erythrocyte, has a life span of approximately 3 months. To compensate for this massive cellular requirement and short life span, the major blood producing tissues contain vast numbers of erythroid progenitor cells. Erythroid progenitors differentiate progressively from hematopoietic stem cells to committed erythroid progenitors to reticulocytes lacking a nucleus and finally to functionally mature erythrocytes in the circulation. Different erythroid progenitor activity, representative of distinct stages of erythropoiesis, can be observed using semisolid colony assays. Distinct stages of erythroid maturation can also be monitored by flow cytometry. Here, we discuss the range of different technical approaches that are used to identify and quantify erythroid progenitors, with particular focus on the mouse as a model system.

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Author information

Authors and Affiliations

  1. Disciplines of Physiology, Faculty of Medicine and Health, School of Medical Sciences, University of Sydney, Camperdown, NSW, Australia

    Chanukya K. Colonne, Campbell V. McKenzie & Stuart T. Fraser

  2. Disciplines of Anatomy and Histology, Faculty of Medicine and Health, School of Medical Sciences, University of Sydney, Camperdown, NSW, Australia

    Jia Hao Yeo & Stuart T. Fraser

  3. Faculty of Medicine and Health, Bosch Institute, University of Sydney, Camperdown, NSW, Australia

    Stuart T. Fraser

  4. The University of Sydney Nano Institute, University of Sydney, Camperdown, NSW, Australia

    Stuart T. Fraser

Authors
  1. Chanukya K. Colonne
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  2. Jia Hao Yeo
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  3. Campbell V. McKenzie
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  4. Stuart T. Fraser
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Corresponding author

Correspondence to Stuart T. Fraser .

Editor information

Editors and Affiliations

  1. NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia

    Mugdha V. Joglekar

  2. NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia

    Anandwardhan A. Hardikar

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Colonne, C.K., Yeo, J.H., McKenzie, C.V., Fraser, S.T. (2019). Identification and Analysis of Mouse Erythroid Progenitor Cells. In: Joglekar, M., Hardikar, A. (eds) Progenitor Cells. Methods in Molecular Biology, vol 2029. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9631-5_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9631-5_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9630-8

  • Online ISBN: 978-1-4939-9631-5

  • eBook Packages: Springer Protocols

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