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High-Throughput Protein Production and Purification pp 69–91Cite as

High-Throughput Protein Production in Yeast

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Part of the Methods in Molecular Biology book series (MIMB,volume 2025)

Abstract

Yeasts are versatile single-celled fungi that grow to high cell densities on inexpensive media. With well-studied genetics and metabolism and a wealth of knowledge available about their propagation and growth in academic as well as industrial settings, yeasts have long been used for recombinant protein production of isolated proteins and multisubunit complexes. They can be easily adapted to high-throughput protein expression pipelines. Importantly, the outcome from small-scale expression evaluations in high-throughput mode is scalable to laboratory and industrial scales using well-established procedures. In this chapter, we offer a state-of-the-art perspective on currently available high-throughput pipelines for protein production in S. cerevisiae and P. pastoris and discuss future challenges and avenues for improvement.

Key words

  • High throughput (HTP)
  • HTP protein production (HTPP)
  • Yeast
  • Saccharomyces cerevisiae
  • Pichia pastoris
  • Recombinant expression

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Acknowledgments

We gratefully acknowledge the support received during the preparation of this chapter. MCV has received funding from the Spanish Ministerio de Economía y Competitividad (PET2008_0101, CTQ2015-66206-C2-2-R, and SAF2015-72961-EXP), the Regional Government of Madrid (S2017/BMD-3673), and the European Commission (Framework Programme 7 (FP7)) project ComplexINC (Contract No. 279039). Abvance Biotech srl contributed with salaries (FJF). Neither funder had any role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Abvance Biotech srl, Madrid, Spain

    Francisco J. Fernández

  2. Center for Biological Research (CIB-CSIC), Structural and Chemical Biology, Madrid, Spain

    Sara Gómez & M. Cristina Vega

Authors
  1. Francisco J. Fernández
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  2. Sara Gómez
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  3. M. Cristina Vega
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Corresponding authors

Correspondence to Francisco J. Fernández or M. Cristina Vega .

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Editors and Affiliations

  1. Architecture et Fonction des Macromolécules Biologiques (AFMB), Unité Mixte de Recherche (UMR) 7257, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université, Marseille cedex 9, France

    Dr. Renaud Vincentelli

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Fernández, F.J., Gómez, S., Vega, M.C. (2019). High-Throughput Protein Production in Yeast. In: Vincentelli, R. (eds) High-Throughput Protein Production and Purification. Methods in Molecular Biology, vol 2025. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9624-7_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9624-7_4

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