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High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization

Part of the Methods in Molecular Biology book series (MIMB,volume 2025)

Abstract

We present a review of high-throughput techniques for the characterization and quality control of proteins in the course of purification, evaluation, and formulation, based on static and dynamic light scattering. Multi-angle static light scattering (MALS) in combination with rapid, low-volume UHPLC size exclusion chromatography is effective in characterizing key biophysical properties, while dynamic light scattering (DLS) in high-throughput microwell-plate format provides large quantities of data in a short time to screen many conditions, excipients, cell lines, or candidate biotherapeutics.

Key words

  • SEC-MALS
  • Dynamic light scattering
  • Protein quality
  • Aggregation
  • Protein formulation
  • Monoclonal antibodies

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  • DOI: 10.1007/978-1-4939-9624-7_16
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Some, D., Razinkov, V. (2019). High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization. In: Vincentelli, R. (eds) High-Throughput Protein Production and Purification. Methods in Molecular Biology, vol 2025. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9624-7_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9624-7_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9623-0

  • Online ISBN: 978-1-4939-9624-7

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