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High-Throughput Protein Production and Purification pp 335–359Cite as

High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization

Part of the Methods in Molecular Biology book series (MIMB,volume 2025)

Abstract

We present a review of high-throughput techniques for the characterization and quality control of proteins in the course of purification, evaluation, and formulation, based on static and dynamic light scattering. Multi-angle static light scattering (MALS) in combination with rapid, low-volume UHPLC size exclusion chromatography is effective in characterizing key biophysical properties, while dynamic light scattering (DLS) in high-throughput microwell-plate format provides large quantities of data in a short time to screen many conditions, excipients, cell lines, or candidate biotherapeutics.

Key words

  • SEC-MALS
  • Dynamic light scattering
  • Protein quality
  • Aggregation
  • Protein formulation
  • Monoclonal antibodies

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Author information

Authors and Affiliations

  1. Wyatt Technology Corp., Santa Barbara, CA, USA

    Daniel Some

  2. Drug Product Technologies, Amgen, Inc., Thousand Oaks, CA, USA

    Vladimir Razinkov

Authors
  1. Daniel Some
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  2. Vladimir Razinkov
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Corresponding author

Correspondence to Daniel Some .

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Editors and Affiliations

  1. Architecture et Fonction des Macromolécules Biologiques (AFMB), Unité Mixte de Recherche (UMR) 7257, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille Université, Marseille cedex 9, France

    Dr. Renaud Vincentelli

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Some, D., Razinkov, V. (2019). High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization. In: Vincentelli, R. (eds) High-Throughput Protein Production and Purification. Methods in Molecular Biology, vol 2025. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9624-7_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9624-7_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9623-0

  • Online ISBN: 978-1-4939-9624-7

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