High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization

  • Daniel SomeEmail author
  • Vladimir Razinkov
Part of the Methods in Molecular Biology book series (MIMB, volume 2025)


We present a review of high-throughput techniques for the characterization and quality control of proteins in the course of purification, evaluation, and formulation, based on static and dynamic light scattering. Multi-angle static light scattering (MALS) in combination with rapid, low-volume UHPLC size exclusion chromatography is effective in characterizing key biophysical properties, while dynamic light scattering (DLS) in high-throughput microwell-plate format provides large quantities of data in a short time to screen many conditions, excipients, cell lines, or candidate biotherapeutics.

Key words

SEC-MALS Dynamic light scattering Protein quality Aggregation Protein formulation Monoclonal antibodies 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Wyatt Technology Corp.Santa BarbaraUSA
  2. 2.Drug Product Technologies, Amgen, Inc.Thousand OaksUSA

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