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Single-Molecule Mechanochemical Sensing Using DNA Origami Nanostructures

Part of the Methods in Molecular Biology book series (MIMB,volume 2027)


Single-molecule techniques such as fluorescence-based methods offer superior sensitivity in biosensing. By direct coupling of analyte recognition and signal amplification, a new sensing strategy, single-molecule mechanochemical sensing, has demonstrated high signal-to-noise ratio in the detection of chemicals and biochemicals. However, parallel sensing is limited. In this strategy, DNA origami nanoassemblies with different sensing probes are used as templates for multiplexing tasks. Assisted by the mechanochemical reporting units, these templates are capable of simultaneous detection of biological samples such as platelet-derived growth factor (PDGF) and DNA fragments in microfluidic channels. The origami-based strategy therefore increases multitasking compatibility of the mechanochemical sensing.

Key words

  • Single-molecule technique
  • Mechanochemical sensing
  • DNA origami nanostructure
  • Optical tweezers

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  • DOI: 10.1007/978-1-4939-9616-2_14
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We thank the NSF (CHE-1609514 and CHE-1415883) for financial support. DNA origami was prepared by Masayuki Endo and Hiroshi Sugiyama at Kyoto University.

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Correspondence to Hanbin Mao .

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Jonchhe, S., Mao, H. (2019). Single-Molecule Mechanochemical Sensing Using DNA Origami Nanostructures. In: Fitzgerald, J., Fenniri, H. (eds) Biomimetic Sensing. Methods in Molecular Biology, vol 2027. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9615-5

  • Online ISBN: 978-1-4939-9616-2

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