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Proteus mirabilis pp 231–240Cite as

Isolation and Purification of Proteus mirabilis Bacteriophage

Part of the Methods in Molecular Biology book series (MIMB,volume 2021)

Abstract

Bacteriophages specifically targeting different strains of bacteria can be isolated from urban sewage using properly modified enrichment techniques. This chapter provides a detailed protocol for isolation of Proteus mirabilis-specific bacteriophages. Briefly, prefiltered sewage is mixed with double-concentrated tryptic soy broth containing the target strain and incubated. Subsequently, the suspension is spread on phage nutrient agar, and if needed, supplemented with swarming motility inhibitor, for the induction of bacterial growth and phage multiplication. Phages infecting bacteria are identified by plaques (patches of dead bacteria) in the confluent bacterial lawn. A pure phage preparation is obtained by cutting out a single plaque from a double-layer agar plate and subsequent virus propagation five times on a given P. mirabilis strain.

Key words

  • Proteus mirabilis
  • Bacteriophage
  • Bacteriophage isolation
  • Sewage
  • Enrichment technique
  • Double-layer agar method
  • Plaque

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Authors and Affiliations

  1. Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland

    Agnieszka Maszewska & Antoni Różalski

Authors
  1. Agnieszka Maszewska
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  2. Antoni Różalski
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Correspondence to Agnieszka Maszewska .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA

    Ph.D. Melanie M. Pearson

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Maszewska, A., Różalski, A. (2019). Isolation and Purification of Proteus mirabilis Bacteriophage. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9601-8_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9600-1

  • Online ISBN: 978-1-4939-9601-8

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