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Culture Methods for Proteus mirabilis

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Proteus mirabilis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2021))

Abstract

Proteus mirabilis is generally easy to culture, but its tendency to swarm on a wide variety of media can interfere with isolation of single colonies or identification of other species in a sample. Therefore, specialized media may be needed to control swarming or to study the bacteria under chemically defined conditions. Here, methods are described for routine culture of P. mirabilis, isolation of P. mirabilis from mixed cultures, and culture of P. mirabilis on physiologically relevant media.

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References

  1. Drzewiecka D (2016) Significance and roles of Proteus spp. bacteria in natural environments. Microb Ecol 72(4):741–758. https://doi.org/10.1007/s00248-015-0720-6

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. O'Hara CM, Brenner FW, Miller JM (2000) Classification, identification, and clinical significance of Proteus, Providencia, and Morganella. Clin Microbiol Rev 13(4):534–546

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Lane MC, Li X, Pearson MM, Simms AN, Mobley HLT (2009) Oxygen-limiting conditions enrich for fimbriate cells of uropathogenic Proteus mirabilis and Escherichia coli. J Bacteriol 191(5):1382–1392

    Article  CAS  PubMed  Google Scholar 

  4. Belas R, Erskine D, Flaherty D (1991) Transposon mutagenesis in Proteus mirabilis. J Bacteriol 173(19):6289–6293

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Lominski I, Lendrum AC (1942) The effect of surface-active agents on B. proteus. J Pathol Bacteriol 54(4):421–433. https://doi.org/10.1002/path.1700540403

    Article  CAS  Google Scholar 

  6. Sandys GH (1960) A new method of preventing swarming of Proteus sp. with a description of a new medium suitable for use in routine laboratory practice. J Med Lab Technol 17:224–233

    CAS  PubMed  Google Scholar 

  7. Mackey JP, Sandys GH (1966) Diagnosis of urinary infections. Brit Med J 1(5496):1173. https://doi.org/10.1136/bmj.1.5496.1173

    Article  PubMed Central  Google Scholar 

  8. Rauprich O, Matsushita M, Weijer CJ, Siegert F, Esipov SE, Shapiro JA (1996) Periodic phenomena in Proteus mirabilis swarm colony development. J Bacteriol 178(22):6525–6538

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Stock I (2003) Natural antibiotic susceptibility of Proteus spp., with special reference to P. mirabilis and P. penneri strains. J Chemotherapy 15(1):12–26. https://doi.org/10.1179/joc.2003.15.1.12

    Article  CAS  Google Scholar 

  10. Russell FE (1963) Synergism between sulphonamide drugs and antibiotics of the polymyxin group against Proteus sp. in vitro. J Clin Pathol 16(4):362. https://doi.org/10.1136/jcp.16.4.362

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Sud IJ, Feingold DS (1970) Mechanism of polymyxin B resistance in Proteus mirabilis. J Bacteriol 104(1):289–294

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Liaw SJ, Lai HC, Ho SW, Luh KT, Wang WB (2000) Inhibition of virulence factor expression and swarming differentiation in Proteus mirabilis by p-nitrophenylglycerol. J Med Microbiol 49(8):725–731

    Article  CAS  PubMed  Google Scholar 

  13. Senior BW (1978) p-nitrophenylglycerol--a superior antiswarming agent for isolating and identifying pathogens from clinical material. J Med Microbiol 11(1):59–61

    Article  CAS  PubMed  Google Scholar 

  14. Alwen J, Smith DG (1967) A medium to suppress swarming of Proteus species. J Appl Bacteriol 30(2):389–394. https://doi.org/10.1111/j.1365-2672.1967.tb00313.x

    Article  CAS  PubMed  Google Scholar 

  15. Hernandez E, Ramisse F, Cavallo JD (1999) Abolition of swarming of Proteus. J Clin Microbiol 37(10):3435–3435

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Firehammer BD (1987) Inhibition of growth and swarming of Proteus mirabilis and Proteus vulgaris by triclosan. J Clin Microbiol 25(7):1312–1313

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Liaw SJ, Lai HC, Wang WB (2004) Modulation of swarming and virulence by fatty acids through the RsbA protein in Proteus mirabilis. Infect Immun 72(12):6836–6845

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Naylor PGD (1964) The effect of electrolytes or carbohydrates in sodium chloride deficient medium on formation of discrete colonies of Proteus and the influence of these substances on growth in liquid culture. J Appl Bacteriol 27(3):422–431. https://doi.org/10.1111/j.1365-2672.1964.tb05050.x

    Article  CAS  Google Scholar 

  19. Armbruster CE, Hodges SA, Mobley HLT (2013) Initiation of swarming motility by Proteus mirabilis occurs in response to specific cues present in urine and requires excess L-glutamine. J Bacteriol 195(6):1305–1319. https://doi.org/10.1128/JB.02136-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Stickler DJ, Morris NS, Winters C (1999) Simple physical model to study formation and physiology of biofilms on urethral catheters. Methods Enzymol 310:494–501

    Article  CAS  PubMed  Google Scholar 

  21. Griffith DP, Musher DM, Itin C (1976) Urease. The primary cause of infection-induced urinary stones. Investig Urol 13(5):346–350

    CAS  Google Scholar 

  22. Brooks T, Keevil CW (1997) A simple artificial urine for the growth of urinary pathogens. Lett Appl Microbiol 24(3):203–206

    Article  CAS  PubMed  Google Scholar 

  23. Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P (ed) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 642–647

    Google Scholar 

  24. Fildes P (1938) The growth of Proteus on ammonium lactate plus nicotinic acid. Br J Exp Pathol 19(4):239–244

    CAS  PubMed Central  Google Scholar 

  25. Neidhardt FC, Bloch PL, Smith DF (1974) Culture medium for enterobacteria. J Bacteriol 119(3):736–747

    CAS  PubMed  PubMed Central  Google Scholar 

  26. MacConkey AT (1908) Bile salt media and their advantages in some bacteriological examinations. J Hyg (Lond) 8(3):322–334

    Article  CAS  Google Scholar 

  27. Holt JG, Krieg NR (1994) Enrichment and isolation. In: Gerhardt P (ed) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, p 205

    Google Scholar 

  28. Hayward NJ, Miles AA (1943) Inhibition of Proteus in cultures from wounds. Lancet 2:116–117

    Article  Google Scholar 

  29. Wang WB, Lai HC, Hsueh PR, Chiou RY, Lin SB, Liaw SJ (2006) Inhibition of swarming and virulence factor expression in Proteus mirabilis by resveratrol. J Med Microbiol 55(Pt 10):1313–1321. https://doi.org/10.1099/jmm.0.46661-0

    Article  CAS  PubMed  Google Scholar 

  30. Alteri CJ, Himpsl SD, Engstrom MD, Mobley HLT (2012) Anaerobic respiration using a complete oxidative TCA cycle drives multicellular swarming in Proteus mirabilis. mBio 3(6). https://doi.org/10.1128/mBio.00365-12

  31. Pearson MM, Yep A, Smith SN, Mobley HLT (2011) Transcriptome of Proteus mirabilis in the murine urinary tract: virulence and nitrogen assimilation gene expression. Infect Immun 79(7):2619–2631. https://doi.org/10.1128/IAI.05152-11

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Mobley HLT (2000) Virulence of the two primary uropathogens - Escherichia coli and Proteus mirabilis exhibit distinct mechanisms of pathogenesis when causing urinary tract infections. ASM News 66(7):403–410

    Google Scholar 

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Acknowledgments

Thank you to Stephanie Himpsl for constructive discussions and Chelsie Armbruster for refining the urine agar method.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA

    Melanie M. Pearson

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  1. Melanie M. Pearson
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Correspondence to Melanie M. Pearson .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA

    Melanie M. Pearson

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Pearson, M.M. (2019). Culture Methods for Proteus mirabilis. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9601-8_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9600-1

  • Online ISBN: 978-1-4939-9601-8

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