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Immunoproteomics pp 137–152Cite as

Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2024))

Abstract

The luciferase immunoprecipitation system (LIPS) assay is a liquid-phase immunoassay that quantitates antigen-specific serum antibodies by measuring luminescence emitted by the reporter enzyme Renilla luciferase (Ruc) fused to an antigen of interest. The LIPS assay can be utilized as a high-throughput and sensitive serological method for profiling serum antibodies recognizing diverse antigens. This chapter provides a detailed protocol for detecting human norovirus-specific serum antibodies with the LIPS assay.

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Acknowledgments

This research was funded by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health. We thank Dr. Peter Burbelo for providing the pRen2 plasmid and Dr. Kim Green for helpful discussion of the manuscript.

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Authors and Affiliations

  1. Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Christine M. Tin & Stanislav V. Sosnovtsev

Authors
  1. Christine M. Tin
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  2. Stanislav V. Sosnovtsev
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Corresponding author

Correspondence to Stanislav V. Sosnovtsev .

Editor information

Editors and Affiliations

  1. Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, ON, Canada

    Kelly M. Fulton

  2. Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, ON, Canada

    Susan M. Twine

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Tin, C.M., Sosnovtsev, S.V. (2019). Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay. In: Fulton, K., Twine, S. (eds) Immunoproteomics. Methods in Molecular Biology, vol 2024. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9597-4_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9597-4_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9596-7

  • Online ISBN: 978-1-4939-9597-4

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