Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis

  • Timothy D. VeenstraEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2024)


The ability to cure or manage many diseases is highly dependent on the ability to correctly diagnose them at the earliest possible stage. Diagnosis relies heavily on biomarkers whether these be visual symptoms or molecules found within samples acquired from the patient. For conditions that lack useful biomarkers, researchers are often faced with the task of sifting through very complex biological samples (i.e., serum, plasma, urine, tissue, cells, etc.) with the hope of discovering a small number of molecules that are exquisitely diagnostic for the condition of interest. One discovery strategy that has been frequently used is to fractionate the biological samples being studied into simpler aliquots that can be more easily characterized using existing technologies. One such fractionation method is to isolate a specific portion based on a specific property (i.e., size, phosphorylation state, charge, etc.) of the proteins within the sample. This method provides a simplified sample that can be characterized at a higher coverage level than the complex sample from which it was derived. This chapter details one of these methods, the extraction and analysis of the low molecular weight proteome of human serum.

Key words

Low molecular weight proteome Mass spectrometry Serum Protein depletion Biomarkers 



This project has been funded in whole or in part with funds from Maranatha Baptist University. The mention of trade names, commercial products, or organizations does not imply endorsement by Maranatha Baptist University.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Applied SciencesMaranatha Baptist UniversityWatertownUSA

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