Abstract
CRISPR/Cas9, an RNA-guided targeted genome editing system, can make precise, targeted modifications to the genome in living cells. Here we describe how this method can be used to efficiently edit the vaccinia virus genome enabling the insertion of transgene(s) specifically into a targeted site.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Cooney EL, Collier AC, Greenberg PD, Coombs RW, Zarling J, Arditti DE et al (1991) Safety of and immunological response to a recombinant vaccinia virus vaccine expressing HIV envelope glycoprotein. Lancet 337:567–572
Di Paola RS, Chen Y, Bubley GJ, Stein MN, Hahn NM, Carducci MA et al (2014) A national multicenter phase 2 study of prostate-specific antigen (PSA) pox virus vaccine with sequential androgen ablation therapy in patients with PSA progression: ECOG 9802. Eur Urol 68(3):372–373
Guo ZS, Bartlett DL (2004) Vaccinia as a vector for gene delivery. Expert Opin Biol Ther 4:901–917
Moss B (2013) Reflections on the early development of poxvirus vectors. Vaccine 31:4220–4222
Nakano E, Panicali D, Paoletti E (1982) Molecular genetics of vaccinia virus: demonstration of marker rescue. Proc Natl Acad Sci U S A 79:1593–1596
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N et al (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339:819–823
Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816–821
Yuan M, Zhang W, Wang J, Al Yaghchi C, Ahmed J, Chard L et al (2015) Efficiently editing the vaccinia virus genome by using the CRISPR-Cas9 system. J Virol 89:5176–5179
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Di Gioia, C., Yuan, M., Wang, Y. (2019). Vaccinia Virus Genome Editing Using CRISPR. In: Mercer, J. (eds) Vaccinia Virus. Methods in Molecular Biology, vol 2023. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9593-6_6
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9593-6_6
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9592-9
Online ISBN: 978-1-4939-9593-6
eBook Packages: Springer Protocols