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Vaccinia Virus pp 255–268Cite as

Super-resolution Microscopy of Vaccinia Virus Particles

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2023))

Abstract

Super-resolution microscopy enables the study of vaccinia architecture at subviral resolution with molecular specificity. Here, we outline how to use structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) to detect fluorescently tagged or immunolabeled viral proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle averaging or quantitative analysis of viral protein spatial organization.

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Acknowledgments

R.G. is funded by the Engineering and Physical Sciences Research Council (EP/M506448/1). D.A. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 750673.

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Author notes

  1. Robert Gray and David Albrecht contributed equally to this work.

Authors and Affiliations

  1. MRC Laboratory for Molecular Cell Biology, University College London, London, UK

    Robert Gray & David Albrecht

Authors
  1. Robert Gray
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  2. David Albrecht
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Corresponding authors

Correspondence to Robert Gray or David Albrecht .

Editor information

Editors and Affiliations

  1. MRC-Laboratory for Molecular Cell Biology, University College London, London, UK

    Jason Mercer

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Gray, R., Albrecht, D. (2019). Super-resolution Microscopy of Vaccinia Virus Particles. In: Mercer, J. (eds) Vaccinia Virus. Methods in Molecular Biology, vol 2023. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9593-6_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9593-6_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9592-9

  • Online ISBN: 978-1-4939-9593-6

  • eBook Packages: Springer Protocols

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