Colony Formation: An Assay of Hematopoietic Progenitor Cells
The colony-forming cell (CFC) assay is used to study the proliferation and differentiation pattern of each input hematopoietic progenitors by their ability to form colonies in a semisolid medium. The resulting colonies are consisting of more differentiated cells, and the number and the morphology of the colonies provide preliminary information about the ability of progenitors to differentiate and proliferate. To allow colonies to grow to a size which facilitates accurate counting and identification, about 14 days of culture is sufficient. In certain situations also shorter periods may be used.
Key wordsFicoll Red blood cell depletion Hematopoietic progenitors Methyl cellulose Semisolid culture Colony-forming units Erythroid and myeloid colonies STEMvision
- 4.Özgüner M, Tavil B, Köksal J, Canal E, Bozkaya I, Tunç B (2011) Comparison of colony forming unit-assay results of different hematopoietic stem cell sources. Turkish J Pediatr Dis 5(4):197–201Google Scholar
- 13.Eaves C (1995) Assays of hematopoietic progenitor cells. In: Beutler E, Lichtman MA, Coller BS, Kipps TJ (eds) Williams hematology, 5th edn. McGraw-Hill, New York, pp 22–66Google Scholar
- 14.Klug CA, Jordan CT (eds) (2002) Hematopoietic stem cell protocols, vol 63. Humana Press, Totowa, NJGoogle Scholar