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Stem Cell Mobilization pp 193–203Cite as

Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays

Part of the Methods in Molecular Biology book series (MIMB,volume 2017)

Abstract

Healthy hematopoietic stem cells (HSCs) are capable to self-renew and reconstitute the complete hematopoietic system. Upon aging, there is an increased incidence of blood-related diseases. Age-related phenotypes have been widely studied by bone marrow transplantation experiments, where reconstitution of the transplanted cells is a direct measure of HSC activity. In this protocol we describe a competitive bone marrow transplantation assay to functionally test young and old HSCs.

Key words

  • Hematopoietic stem cells
  • Transplants
  • Young
  • Aged
  • Bone marrow
  • Competitive

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Author information

Authors and Affiliations

  1. Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany

    Yu Wei Zhang & Nina Cabezas-Wallscheid

  2. International Max Planck Research School for Molecular and Cellular Biology (IMPRS-MCB), Freiburg, Germany

    Yu Wei Zhang

Authors
  1. Yu Wei Zhang
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  2. Nina Cabezas-Wallscheid
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Corresponding author

Correspondence to Nina Cabezas-Wallscheid .

Editor information

Editors and Affiliations

  1. Department of Internal Medicine II, Center for Medical Research, University of Tübingen, Tübingen, Germany

    Gerd Klein

  2. Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany

    Patrick Wuchter

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Zhang, Y.W., Cabezas-Wallscheid, N. (2019). Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays. In: Klein, G., Wuchter, P. (eds) Stem Cell Mobilization. Methods in Molecular Biology, vol 2017. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9574-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9574-5_15

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  • Publisher Name: Humana, New York, NY

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  • Online ISBN: 978-1-4939-9574-5

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