Application of Whole-Genome Sequencing to Transposon Mutants of Salmonella Heidelberg

  • Bryna Rackerby
  • Sang In Lee
  • Ian Moppert
  • Steven C. Ricke
  • KwangCheol C. Jeong
  • Si Hong ParkEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2016)


Transposons are elements widely dispersed among organisms which are able to move and replicate fragments of genomes. The extensive variability in transposons present in most organisms requires extensive identification and interpretation of the resulting transposon mutants after transposon mutagenesis. However, much of this is reliant on utilizing randomness characteristics of transposon to identify essential genes for the organism of interest. Integration of the transposon mutant approach with commercialized next-generation sequencing (NGS) technology has helped to advance transposon identification by sequencing millions of reads generated from a single run on an NGS platform. Transposon sequencing is defined as a gene sequencing methodology that allows for the identification of nonessential genes and the determination of gene function using a random transposon insertional mutagenesis followed by massively parallel sequencing. The detailed protocol will be outlined in this chapter. The genomic DNA integrated with the transposons is sequenced using an NGS platform in order to determine the location of each mutation.

Key words

Salmonella Heidelberg Transposon mutant Next-generation sequencing (NGS) Whole-genome sequencing (WGS) 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Bryna Rackerby
    • 1
  • Sang In Lee
    • 2
  • Ian Moppert
    • 2
  • Steven C. Ricke
    • 3
    • 4
  • KwangCheol C. Jeong
    • 5
  • Si Hong Park
    • 2
    Email author
  1. 1.Department of MicrobiologyOregon State UniversityCorvallisUSA
  2. 2.Department of Food Science and TechnologyOregon State UniversityCorvallisUSA
  3. 3.Department of Food Science, Center for Food SafetyUniversity of ArkansasFayettevilleUSA
  4. 4.Cell and Molecular Biology ProgramUniversity of ArkansasFayettevilleUSA
  5. 5.Department of Animal Science, Institute of Food and Agricultural SciencesUniversity of FloridaGainesvilleUSA

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