Abstract
Tissue culture systems can be powerful tools for studying the process of cell differentiation in detail. Although a large number of cultures for xylem differentiation have been developed and utilized, there are only few reports on culture systems for ectopic phloem differentiation. Recently, a novel tissue culture system named Vascular cell Induction culture System Using Arabidopsis Leaves (VISUAL) was established, in which both xylem and phloem cells can be rapidly and efficiently induced in the model plant Arabidopsis thaliana. This chapter discusses the principle of VISUAL and how it can be used to investigate phloem differentiation, for example in combination with genetic experiments or transcriptome analysis. In addition, the protocol for establishing a phloem cell culture is provided.
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References
Anne P, Hardtke CS (2018) Phloem function and development-biophysics meets genetics. Curr Opin Plant Biol 43:11–28
Heo JO, Roszak P, Furuta KM, Helariutta Y (2014) Phloem development: current knowledge and future perspectives. Am J Bot 101:1393–1402
Saito M, Nurani AM, Kondo Y, Fukuda H (2017) Tissue culture for xylem differentiation with Arabidopsis leaves. Methods Mol Biol 1544:59–65
Nurani AM, Kondo Y, Fukuda H (2018) Ectopic xylem and phloem induction in Arabidopsis cotyledons for sequential analysis of vascular differentiation. Methods Mol Biol 1830:149–159
Kondo Y, Ito T, Nakagami H, Hirakawa Y, Saito M, Tamaki T, Shirasu K, Fukuda H (2014) Plant GSK3 proteins regulate xylem cell differentiation downstream of TDIF-TDR signalling. Nat Commun 5:3504
Kondo Y, Fujita T, Sugiyama M, Fukuda H (2015) A novel system for xylem cell differentiation in Arabidopsis thaliana. Mol Plant 8:612–621
Kondo Y, Nurani AM, Saito C, Ichihashi Y, Saito M, Yamazaki K, Mitsuda N, Ohme-Takagi M, Fukuda H (2016) Vascular cell induction culture system using Arabidopsis leaves (VISUAL) reveals the sequential differentiation of sieve element-like cells. Plant Cell 28:1250–1262
Furuta KM, Yadav SR, Lehesranta S, Belevich I, Miyashima S, Heo JO, Vatén A, Lindgren O, De Rybel B, Van Isterdael G, Somervuo P, Lichtenberger R, Rocha R, Thitamadee S, Tähtiharju S, Auvinen P, Beeckman T, Jokitalo E, Helariutta Y (2014) Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation. Science 345:933–937
Breda AS, Hazak O, Hardtke CS (2017) Phosphosite charge rather than shootward localization determines OCTOPUS activity in root protophloem. Proc Natl Acad Sci U S A 114:E5721–E5730
Bonke M, Thitamadee S, Mähönen AP, Hauser MT, Helariutta Y (2003) APL regulates vascular tissue identity in Arabidopsis. Nature 426:181–186
Wallner ES, López-Salmerón V, Belevich I, Poschet G, Jung I, Grünwald K, Sevilem I, Jokitalo E, Hell R, Helariutta Y, Agustà J, Lebovka I, Greb T (2017) Strigolactone- and karrikin-independent SMXL proteins are central regulators of phloem formation. Curr Biol 27:1241–1247
Wu YY, Hou BH, Lee WC, Lu SH, Yang CJ, Vaucheret H, Chen HM (2017) DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation. Plant J 90:1064–1078
Saito M, Kondo Y, Fukuda H (2018) BES1 and BZR1 redundantly promote phloem and xylem differentiation. Plant Cell Physiol 59:590–600
Acknowledgments
We thank Dr. Yka Helariutta and Dr. Hiroo Fukuda for the kind support of our research. This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (17H06476 to Y.K.), and from the Japan Society for the Promotion of Science (17H05008 to Y.K.).
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Saito, M., Kondo, Y. (2019). What Can Cell Culture Systems Reveal About Sieve Element Differentiation?. In: Liesche, J. (eds) Phloem. Methods in Molecular Biology, vol 2014. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9562-2_36
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DOI: https://doi.org/10.1007/978-1-4939-9562-2_36
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