Phloem pp 411-420 | Cite as

Efficient Establishment of Interfamily Heterograft of Nicotiana benthamiana and Arabidopsis thaliana

  • Koji Okayasu
  • Michitaka NotaguchiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)


The grafting technique has been applied to study systemic signaling in plants, especially to investigate whether gene action is graft transmissible and/or gene products such as RNAs and proteins are transported systemically. Here we describe an interfamily heterograft system between Nicotiana benthamiana scion plants and Arabidopsis stock plants for the identification of systemic phloem-mobile signals. Since these plants belong to evolutionary distant families and genome databases are available for both, we can reliably identify mobile substances transported from one to the other plant.

Key words

Grafting Interfamily grafting Heterograft Wedge-graft Nicotiana Arabidopsis Mobile signals Phloem transport 



We thank Dr. Johannes Liesche for careful editing of the manuscript. This work was supported by JSPS grant 18H04778; JST PRESTO grant JPMJPR15O3; MAFF Science and Technology Research Promotion Program for agriculture, forestry, fisheries and food industry grant 16770567; and MEXT grant 16811669 to M.N.


  1. 1.
    Hartmann HT, Kester DE (1975) Plant propagation: principles and practices, 3rd edn. Prentice Hall, Englewood Cliffs, NJGoogle Scholar
  2. 2.
    Mudge K, Janick J, Scofield S, Goldschmidt EE (2009) A history of grafting. In: Janick J (ed) Horticultural reviews, vol 35. Wiley, Hoboken, pp 437–493CrossRefGoogle Scholar
  3. 3.
    Goldschmidt EE (2014) Plant grafting: new mechanisms, evolutionary implications. Front Plant Sci 5:727CrossRefGoogle Scholar
  4. 4.
    Tsutsui H, Notaguchi M (2017) The use of grafting to study systemic signaling in plants. Plant Cell Physiol 58:1291–1301CrossRefGoogle Scholar
  5. 5.
    Notaguchi M, Okamoto S (2015) Dynamics of long-distance signaling via plant vascular tissues. Front Plant Sci 6:161CrossRefGoogle Scholar
  6. 6.
    Spiegelman Z, Golan G, Wolf S (2013) Don’t kill the messenger: long-distance trafficking of mRNA molecules. Plant Sci 213:1–8CrossRefGoogle Scholar
  7. 7.
    Thieme CJ, Rojas-Triana M, Stecyk E, Schudoma C, Zhang W, Yang L et al (2015) Endogenous Arabidopsis messenger RNAs transported to distant tissues. Nat Plants 1:15025CrossRefGoogle Scholar
  8. 8.
    Yang Y, Mao L, Jittayasothorn Y, Kang Y, Jiao C, Fei Z et al (2015) Messenger RNA exchange between scions and rootstocks in grafted grapevines. BMC Plant Biol 15:251CrossRefGoogle Scholar
  9. 9.
    Zhang Z, Zheng Y, Ham BK, Chen J, Yoshida A, Kochian LV et al (2016) Vascular-mediated signalling involved in early phosphate stress response in plants. Nat Plants 2:16033CrossRefGoogle Scholar
  10. 10.
    Lin MK, Belanger H, Lee YJ, Varkonyi-Gasic E, Taoka K, Miura E et al (2007) FLOWERING LOCUS T protein may act as the long-distance florigenic signal in the cucurbits. Plant Cell 19:1488–1506CrossRefGoogle Scholar
  11. 11.
    Notaguchi M, Wolf S, Lucas WJ (2012) Phloem-mobile Aux/IAA transcripts target to the root tip and modify root architecture. J Integr Plant Biol 54:760–772CrossRefGoogle Scholar
  12. 12.
    Notaguchi M, Higashiyama T, Suzuki T (2015) Identification of mRNAs that move over long distances using an RNA-Seq analysis of Arabidopsis/Nicotiana benthamiana heterografts. Plant Cell Physiol 56:311–321CrossRefGoogle Scholar
  13. 13.
    Arabidopsis Biological Resource Center (2013) The ABRC register. Accessed 13 May 2018
  14. 14.
    Lee JM, Oda M (2003) Grafting of herbaceous vegetables and ornamental crops. In: Janick J (ed) Horticultural reviews, vol 28. Wiley, Hoboken, pp 61–124Google Scholar
  15. 15.
    Notaguchi M, Daimon Y, Abe M, Araki T (2009) Adaptation of a seedling micro-grafting technique to the study of long-distance signaling in flowering of Arabidopsis thaliana. J Plant Res 122:201–214CrossRefGoogle Scholar
  16. 16.
    Melnyk CW, Schuster C, Leyser O, Meyerowitz EM (2015) A developmental framework for graft formation and vascular reconnection in Arabidopsis thaliana. Curr Biol 25:1306–1318CrossRefGoogle Scholar
  17. 17.
    Matsuoka K, Sugawara E, Aoki R, Takuma K, Terao-Morita M, Satoh S et al (2016) Differential cellular control by cotyledon-derived phytohormones involved in graft reunion of Arabidopsis hypocotyls. Plant Cell Physiol 57:2620–2631CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan

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