Abstract
Diagnosing malaria is a key component of effective case management and monitoring of antimalarial programs worldwide. This chapter features the different diagnostic approaches currently in use or under testing for use in case management and/or epidemiological studies of malaria. Emphasis is laid on the basic principles of each diagnostic approach as well as their operational limits under different malaria endemicity settings. The discussed methods are defined as “conventional” or “unconventional” depending on their widespread use in malaria case management. The chapter therefore provides a practical guide to students, health practitioners, and field researchers involved in the fight against malaria through community-based interventions.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Koram KA, Molyneux ME (2007) When is “malaria” malaria? The different burdens of malaria infection, malaria disease, and malaria-like illnesses. Am J Trop Med Hyg 77(6 Suppl):1–5
Wilson ML (2013) Laboratory diagnosis of malaria conventional and rapid diagnostic methods. Arch Pathol Lab Med 137:805–811
Chandramohan D, Jaffar S, Greenwood B (2002) Use of clinical algorithms for diagnosing malaria. Tropical Med Int Health 7:45–52
Ahmed MU, Hossain MA, Khan AH et al (2013) Efficacy of immunodiagnosis of falciparum malaria at different levels of parasitemia. J Enam Med Col 3:88–90
Ali IM, Bigoga JD, Forsah DA et al (2016) Field evaluation of the 22 rapid diagnostic tests for community management of malaria with artemisinin combination therapy in Cameroon. Malar J 15:31
Hendriksen IC, Mtove G, Pedro AJ et al (2011) Evaluation of a PfHRP2 and a pLDH-based rapid diagnostic test for the diagnosis of severe malaria in 2 populations of African children. Clin Infect Dis 52:1100–1107
Britton S, Cheng Q, McCarthy JS (2016) Novel molecular diagnostic tools for malaria elimination: a review of options from the point of view of high-throughput and applicability in resource limited settings. Malar J 15:88
Khan SA, Ahmed S, Mushahid N et al (2013) Comparison of real time polymerase chain reaction with microscopy and antigen detection assay for the diagnosis of malaria. J Coll Physicians Surg Pak 23:787–792
Chander Y, Koelbl J, Puckett J et al (2014) A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification (LAMP). Front Microbiol 5:395
Kemleu S, Guelig D, Eboumbou MC et al (2016) A field-tailored reverse transcription loop-mediated isothermal assay for high sensitivity detection of plasmodium falciparum infections. PLoS One 11:e0165506
Kasetsirikul S, Buranapong J, Srituravanich W et al (2016) The development of malaria diagnostic techniques: a review of the approaches with focus on dielectrophoretic and magnetophoretic methods. Malar J 15:358
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Ayong, L., Moukoko, C.E.E., Mbacham, W.F. (2019). Diagnosing Malaria: Methods, Tools, and Field Applicability. In: Ariey, F., Gay, F., Ménard, R. (eds) Malaria Control and Elimination. Methods in Molecular Biology, vol 2013. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9550-9_5
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9550-9_5
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9549-3
Online ISBN: 978-1-4939-9550-9
eBook Packages: Springer Protocols