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Antibody-Driven Proximity Labeling in Fixed Tissues

  • Daniel Z. Bar
  • Francis S. CollinsEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2008)

Abstract

Protein function often depends on assemblies and interactions. These show complex spatial and temporal organization within the cell. Analysis of protein function can be greatly assisted by grouping proteins with their neighbors. Rather than relying on affinity, proximity labeling targets proteins proximal to the target of interest. We describe a protocol for antibody-guided deposition of tags in fixed and permeabilized cell lines and primary human tissue samples.

Key words

Proximity labeling Antibody Biotin HRP Mass-spectrometry Protein-protein interactions 

Notes

Acknowledgments

We thank L. Bonnycastle and A. Thatavarty for useful suggestions and comments. This work was supported by intramural NIH funding, project ZIA HG200305-15.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.The National Human Genome Research Center, National Institute of HealthBethesdaUSA
  2. 2.Department of Oral Biology, The Goldschleger School of Dental Medicine, Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael

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