Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System
The cohesin complex is required to establish sister chromatid cohesion and ensure accurate chromosome segregation after DNA replication. Recent data has also revealed a role for cohesion as a major player in DNA repair and gene expression regulation. All subunits of the Cohesin complex are essential and cannot be deleted. Here, we describe a protocol to efficiently deplete cohesin subunits with an auxin-inducible degron (AID) system in S. cerevisiae.
Key wordsS. cerevisiae Cohesin Auxin degron
This work was supported by a grant from the European Research Council under the European Community’s Seventh Framework Program (FP7/2007 2013/European Research Council grant agreement 281287) and fundings from the Radiobiology program of the CEA Segment 4 and ANR (DICENs-ANR-14-CE10-0021-01).
- 3.Nasmyth K (2001) Disseminating the genome: joining, resolving, and separating sister chromatids during mitosis and meiosis. Annu Rev Genet 35:673–745. https://doi.org/10.1146/annurev.genet.35.102401.091334 CrossRefPubMedGoogle Scholar
- 7.Nasmyth K, Haering CH (2005) The structure and function of SMC and kleisin complexes. Annu Rev Biochem 74:595–648. https://doi.org/10.1146/annurev.biochem.74.082803.133219 CrossRefPubMedGoogle Scholar