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Physicochemical Characterization of Phthalocyanine-Functionalized Quantum Dots by Capillary Electrophoresis Coupled to a LED Fluorescence Detector

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Pharmaceutical Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2000))

Abstract

Capillary zone electrophoresis (CZE) complemented with Taylor Dispersion Analysis-CE (TDA-CE) was developed to physicochemically characterize phthalocyanine-capped core/shell/shell quantum dots (QDs) at various pH and ionic strengths. An LED-induced fluorescence detector was used to specifically detect the QDs. The electropherograms and taylorgrams allowed calculating the phthalocyanine-QDs (Pc-QDs) ζ-potential and size, respectively, and determining the experimental conditions for colloidal stability. This methodology allowed evidencing either a colloidal stability or an aggregation state according to the background electrolytes nature. The calculated ζ-potential values of Pc-QDs decreased when ionic strength increased, being well correlated with the aggregation of the nanoconjugates at elevated salt concentrations. For the same reason, the hydrodynamic diameter of Pc-QDs increased with increasing background electrolyte ionic strength. The use of electrokinetic methodologies has provided insights into the colloidal stability of the photosensitizer-functionalized QDs in physiologically relevant solutions and, thereby, its usefulness for improving their design and applications for photodynamic therapy.

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Acknowledgments

This work was partially supported by the Department of Science and Technology (DST) and National Research Foundation (NRF) of South Africa, through the DST/NRF South African Research Chairs Initiative for Professor of Medicinal Chemistry and Nanotechnology (UID = 62620) and Rhodes University and by DST/Mintek Nanotechnology Innovation Centre (NIC). GRG is grateful to the Mexican National Council for Science and Technology (CONACYT). The authors acknowledge financial support from PROTEA Project 33885ZJ (France–South Africa).

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Authors and Affiliations

  1. Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé UTCBS, Paris, France

    Gonzalo Ramírez-García, Fanny d’Orlyé, Fethi Bedioui & Anne Varenne

  2. INSERM, UTCBS U 1022, Paris, France

    Gonzalo Ramírez-García, Fanny d’Orlyé, Fethi Bedioui & Anne Varenne

  3. CNRS, UTCBS UMR 8258, Paris, France

    Gonzalo Ramírez-García, Fanny d’Orlyé, Fethi Bedioui & Anne Varenne

  4. Université Paris Descartes, Sorbonne Paris Cité, UTCBS, Paris, France

    Gonzalo Ramírez-García, Fanny d’Orlyé, Fethi Bedioui & Anne Varenne

  5. Department of Chemistry, Rhodes University, Grahamstown, South Africa

    Gonzalo Ramírez-García & Tebello Nyokong

Authors
  1. Gonzalo Ramírez-García
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  2. Fanny d’Orlyé
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  3. Tebello Nyokong
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  4. Fethi Bedioui
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  5. Anne Varenne
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Corresponding author

Correspondence to Anne Varenne .

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Editors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, Midwestern University, Glendale, AZ, USA

    Volkmar Weissig

  2. Nanomedicine Center of Excellence in Translational Cancer Research, Midwestern University, Glendale, AZ, USA

    Tamer Elbayoumi

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Ramírez-García, G., d’Orlyé, F., Nyokong, T., Bedioui, F., Varenne, A. (2019). Physicochemical Characterization of Phthalocyanine-Functionalized Quantum Dots by Capillary Electrophoresis Coupled to a LED Fluorescence Detector. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_23

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  • DOI: https://doi.org/10.1007/978-1-4939-9516-5_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9515-8

  • Online ISBN: 978-1-4939-9516-5

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