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Metabolomics pp 199–206Cite as

Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1996))

Abstract

Lipid droplets (LDs) are dynamic organelles that regulate the storage and homeostasis of intracellular triglycerides and other neutral lipids. Studies show that the number, morphology, and subcellular localization of LDs are altered in a growing number of diseases. As such, methodologies for imaging and quantifying LDs have become essential tools for detecting changes in cellular lipid metabolism, which could be an important indicator of disease onset or progression. We previously reported on the accumulation of LDs in podocytes of the kidney glomerulus in nephrological diseases of metabolic and non-metabolic origin. Here, we describe a high-content analysis (HCA) method for automated detection and quantification of LDs in differentiated human podocytes.

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Author information

Author notes

  1. Shamroop Kumar Mallela and Devang Maheshkumar Patel contributed equally to this chapter.

Authors and Affiliations

  1. Katz Family Division of Nephrology and Hypertension, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA

    Shamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra Merscher, Alessia Fornoni & Hassan Al-Ali

  2. Peggy and Harold Katz Family Drug Discovery Center, University of Miami Miller School of Medicine, Miami, FL, USA

    Shamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra Merscher, Alessia Fornoni & Hassan Al-Ali

  3. Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, FL, USA

    Hassan Al-Ali

  4. The Miami Project to Cure Paralysis, University of Miami, Miller School of Medicine, Miami, FL, USA

    Hassan Al-Ali

Authors
  1. Shamroop Kumar Mallela
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  2. Devang Maheshkumar Patel
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  3. Gloria Michelle Ducasa
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  4. Sandra Merscher
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  5. Alessia Fornoni
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  6. Hassan Al-Ali
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Corresponding authors

Correspondence to Alessia Fornoni or Hassan Al-Ali .

Editor information

Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA

    Sanjoy K. Bhattacharya

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Mallela, S.K., Patel, D.M., Ducasa, G.M., Merscher, S., Fornoni, A., Al-Ali, H. (2019). Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9488-5_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9487-8

  • Online ISBN: 978-1-4939-9488-5

  • eBook Packages: Springer Protocols

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