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Metabolomics pp 131–153Cite as

Analysis of Microbial Siderophores by Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1996))

Abstract

Siderophores represent important microbial virulence factors and infection biomarkers. Their monitoring in fermentation broths, bodily fluids, and tissues should be reproducible. Similar isolation, characterization, and quantitation studies can often have conflicting results, and without proper documentation of sample collection, data processing, and analysis methods, it is difficult to reexamine the data and reconcile these differences. In this Springer Nature Protocol, we present the procedure optimized for ferricrocin/triacetylfusarinine C extraction from biological material as well as for tissue fixation and cryosectioning for optical microscopy and for both elemental and molecular mass spectrometry imaging. Special attention is paid to siderophore data mining from conventional and product ion mass spectra, liquid chromatography, and mass spectrometry imaging datasets, performed here by our free software called CycloBranch.

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Acknowledgments

This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (LO1509) and Czech Science Foundation (19-10907S).

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Authors and Affiliations

  1. Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic

    Tomas Pluhacek, Anton Skriba, Jiri Novak, Dominika Luptakova & Vladimir Havlicek

Authors
  1. Tomas Pluhacek
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  2. Anton Skriba
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  3. Jiri Novak
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  4. Dominika Luptakova
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  5. Vladimir Havlicek
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Corresponding author

Correspondence to Vladimir Havlicek .

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Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA

    Sanjoy K. Bhattacharya

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Pluhacek, T., Skriba, A., Novak, J., Luptakova, D., Havlicek, V. (2019). Analysis of Microbial Siderophores by Mass Spectrometry. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9488-5_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9487-8

  • Online ISBN: 978-1-4939-9488-5

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