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Genetic Engineering Approaches Used to Increase Lipid Production and Alter Lipid Profile in Microbes

  • Xiao-Ling Tang
  • Ya-Ping XueEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1995)

Abstract

Microbial production of lipids provides important alternative sources for a variety of fine chemicals and fuels. With the development of biotechnology, genetic engineering approaches are widely used to increase lipid production in microbes, as well as to alter the lipid profile with unique physicochemical properties. In this chapter, based on the well-known information of de novo lipid accumulation mechanisms in microbes, genetic engineering strategies at the direction of increased supply of substrates, regulation of lipid synthesis pathway, regulation of lipid catabolic pathway, and regulation of lipid profiles are described. These methods provide promising insights to promote the optimization of lipid accumulation and properties.

Key words

Microbial lipids Genetic engineering Lipid accumulation Lipid profile alternation 

References

  1. 1.
    Ratledge C (1994) Yeasts, molds, algae and bacteria as sources of lipids. In: Kamel BS, Kakuda Y (eds) Technological advances in improved and alternative sources of lipids. Blackie Academic and Professional, London, pp 235–291CrossRefGoogle Scholar
  2. 2.
    Fujimoto T, Ohsaki Y, Cheng J, Suzuki M, Shinohara Y (2008) Lipid droplets: a classic organelle with new outfits. Histochem Cell Biol 130:263–279CrossRefGoogle Scholar
  3. 3.
    Janßen HJ, Steinbüchel A (2014) Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels. Biotechnol Biofuels 7:7CrossRefGoogle Scholar
  4. 4.
    Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J (2013) Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab Eng 15:48–54CrossRefGoogle Scholar
  5. 5.
    Lian J, Si T, Nair NU, Zhao H (2014) Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains. Metab Eng 24:139–149CrossRefGoogle Scholar
  6. 6.
    Tang X, Chen WN (2014) Investigation of fatty acid accumulation in the engineered Saccharomyces cerevisiae under nitrogen limited culture condition. Bioresour Technol 162:200–206CrossRefGoogle Scholar
  7. 7.
    de Jong BW, Shi S, Siewers V, Nielsen J (2014) Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway. Microb Cell Factories 13:39CrossRefGoogle Scholar
  8. 8.
    Zhang Y, Adams IP, Ratledge C (2007) Malic enzyme: the controlling activity for lipid production? Overexpression of malic enzyme in Mucor circinelloides leads to a 2.5-fold increase in lipid accumulation. Microbiology 153:2013–2025CrossRefGoogle Scholar
  9. 9.
    Runguphan W, Keasling JD (2014) Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals. Metab Eng 21:103–113CrossRefGoogle Scholar
  10. 10.
    Shi S, Chen Y, Siewers V, Nielsen J (2014) Improving production of malonyl coenzyme A- derived metabolites by abolishing Snf1-dependent regulation of Acc1. MBio 5:e01130–e01114PubMedPubMedCentralGoogle Scholar
  11. 11.
    Chen WN, Tan KY (2013) Malonate uptake and metabolism in Saccharomyces cerevisiae. Appl Biochem Biotechnol 171:44–62CrossRefGoogle Scholar
  12. 12.
    Nevoigt E, Kohnke J, Fischer CR, Alper H, Stahl U, Stephanopoulos G (2006) Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiae. Appl Environ Microbiol 72:5266–5273CrossRefGoogle Scholar
  13. 13.
    Mora G, Scharnewski M, Fulda M (2012) Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae. PLoS One 7:e49269CrossRefGoogle Scholar
  14. 14.
    Kamisaka Y, Kimura K, Uemura H, Yamaoka M (2013) Overexpression of the active diacylglycerolacyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast. Appl Microbiol Biotechnol 97:7345–7355CrossRefGoogle Scholar
  15. 15.
    Mličková K, Luo Y, D'Andrea S, Peč P, Chardot T, Nicaud J (2004) Acyl-CoA oxidase, a key step for lipid accumulation in the yeast Yarrowia lipolytica. J Mol Catal B Enzym 28:81–85CrossRefGoogle Scholar
  16. 16.
    Yazawa H, Iwahashi H, Kamisaka Y, Kimura K, Uemura H (2009) Production of polyunsaturated fatty acids in yeast Saccharomyces cerevisiae and its relation to alkaline pH tolerance. Yeast 26:167–184CrossRefGoogle Scholar
  17. 17.
    Chen L, Zhang J, Chen WN (2014) Engineering of Saccharomyces cerevisiae β-oxidation pathway to increase medium chain fatty acid production as potential biofuel. PLoS One 9:e84853CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Zhejiang University of TechnologyHangzhouChina

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