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Quantification of Lipid Content in Oleaginous Biomass Using Thermogravimetry

  • Balakrishna Maddi
  • Agasteswar Vadlamani
  • Sridhar ViamajalaEmail author
  • Sasidhar Varanasi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1995)

Abstract

Laboratory analytical techniques employed for triglyceride quantification in oleaginous biomass (e.g., microalgae and oilseeds) involve multiple steps and typically require use of volatile organic solvents. Here we describe a single-step approach for measurement of triglycerides using thermogravimetry (TG). We have observed that triglycerides undergo complete volatilization over a narrow temperature interval of 370–450 °C, with negligible solid residue under inert atmosphere, whereas other constituents of oleaginous biomass (such as proteins and carbohydrates) primarily degrade below 350 °C. As a result, triglyceride content of biomass can be estimated using TG by determining the mass loss of the sample in the temperature interval of 370–450 °C.

Key words

Microalgae Thermogravimetry Lipids Triglycerides Oleaginous biomass 

Notes

Acknowledgments

This work was supported by (1) the US Department of Energy Bioenergy Technologies Office (award# DE-EE0005993) and (2) the National Science Foundation through the Sustainable Energy Pathways Program (award# CHE-1230609)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Balakrishna Maddi
    • 1
  • Agasteswar Vadlamani
    • 2
  • Sridhar Viamajala
    • 2
    Email author
  • Sasidhar Varanasi
    • 2
  1. 1.Suganit Bio-Renewables LLCToledoUSA
  2. 2.Department of Chemical EngineeringThe University of ToledoToledoUSA

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