Rhodococcus and Yarrowia-Based Lipid Production Using Lignin-Containing Industrial Residues

  • Rosemary K. Le
  • Kristina M. Mahan
  • Arthur J. RagauskasEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1995)


Improvement in biorefining technologies coupled with development of novel fermentation strategies and analysis will be paramount in establishing supplementary and sustainable biofuel pathways. Oleaginous microorganisms that are capable of accumulating triacylglycerides (TAGs) and fatty acid methyl esters (FAMEs), such as Rhodococcus and Yarrowia species, can be used to produce second-generation biofuels from non-food competing carbon sources. These “microbiorefineries” provide a pathway to upgrade agricultural and industrial waste streams to fungible fuels or precursors to chemicals and materials. Here we provide a general overview on cultivating Rhodococcus and Yarrowia on agro-waste/industrial biomass pretreatment waste streams to produce single-cell oils/lipids and preparing samples for FAME detection.

Key words

Yeast Rhodococcus Yarrowia Fermentation Agro-waste Lipid production 



This work was supported by US Department of Energy (award #DE—EE0006112), and we would like to acknowledge our collaborator Joshua S. Yuan at Texas A&M University.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rosemary K. Le
    • 1
  • Kristina M. Mahan
    • 1
  • Arthur J. Ragauskas
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Chemical and Biomolecular EngineeringUniversity of TennesseeKnoxvilleUSA
  2. 2.Biosciences DivisionOak Ridge National LaboratoryOak RidgeUSA
  3. 3.Department of Forestry, Wildlife and Fisheries, Center of Renewable Carbon, Institute of AgricultureUniversity of TennesseeKnoxvilleUSA

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