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Qualitative and Quantitative Estimation of Bacterial Lipid Production

  • Gangatharan MuralitharanEmail author
  • Manickam Gayathri
  • Sumathy Shunmugam
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1995)

Abstract

An ever increasing energy demand and fast depletion of fossil fuels have led to increased consideration of bacterial lipids as a renewable biofuel source. Many methods are available for both physical and chemical extraction of bacterial lipids. The method of choice will depend on the nature of sample to be analyzed, combinations of solvent systems preferred, content and quality of the lipid to be analyzed, types of equipment available, and time of the extraction procedures employed. Here we describe the most reliable, routine method of extracting bacterial lipids and evaluating the growth kinetic parameters like biomass and lipid productivity and lipid content. We also describe the method of comparing bacterial fatty acid methyl ester peaks with standard peaks for analysis.

Key words

Bacteria Cyanobacteria Growth kinetic parameters Lipid extraction Fatty acid methyl ester (FAME) Transesterification 

Notes

Acknowledgments

M.G. acknowledges Bharathidasan University authorities for the University Research Fellowship (05441/URF/K7/2013 dated 04.07.2013). The authors are thankful to DST-FIST program (SR/FIST/LSI/-013/2012 dated 13.08.2012) for instrument facilities.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Gangatharan Muralitharan
    • 1
    Email author
  • Manickam Gayathri
    • 1
  • Sumathy Shunmugam
    • 1
  1. 1.Department of Microbiology, Centre of Excellence in Life SciencesBharathidasan UniversityTiruchirappalliIndia

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