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Impact of Culture Conditions on Neutral Lipid Production by Oleaginous Yeast

  • Irene Fakankun
  • Maryam Mirzaei
  • David B. LevinEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1995)

Abstract

Oleaginous yeasts have the ability to accumulate and store triacylglycerides (TAGs) to more than 20% of their cell mass. Oleaginous yeasts have advantages over oil seed plants and microalgae because they grow much faster (doubling time is usually less than an hour), accumulate cell mass to much higher densities, and are less affected by seasonal or weather conditions. The TAGs synthesized by oleaginous yeasts are often rich in polyunsaturated fatty acids and can be used either for biodiesel production or as edible oils. “Red” yeasts are oleaginous yeasts that can synthesize and accumulate high concentrations of TAGs. Many factors affect the growth of red yeasts and subsequent yields of TAGs. These factors include carbon and nitrogen sources, their concentrations, the C/N ratio, temperature, pH, aeration rate, mineral elements, inorganic salts, and inhibitors. The effect of each factor varies with the yeast strain and its growth phase. Rhodosporidium diobovatum is a “red” yeast that can utilize low-cost substrates, such as waste glycerol derived from biodiesel production as a carbon source, and can synthesize and accumulate high concentrations of both TAGs and carotenoids.

Key words

Oleaginous yeasts Red yeasts Single cell oils Biodiesel production Rhodosporidium diobovatum Triacylglycerides 

Notes

Acknowledgments

This work was supported by the Natural Sciences and Engineering Research Council (NSERC) of Canada through an NSERC Discovery Grant (award #: RGPIN 05961-16).

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Authors and Affiliations

  • Irene Fakankun
    • 1
  • Maryam Mirzaei
    • 1
  • David B. Levin
    • 1
    Email author
  1. 1.Department of Biosystems EngineeringUniversity of ManitobaWinnipegCanada

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