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A Protocol for One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes

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Cell-Based Assays Using iPSCs for Drug Development and Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1994))

Abstract

Within the glomerulus, podocytes are highly specialized visceral epithelial cells that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the nonproliferative state of the cells. In addition, rapid dedifferentiation is often observed. Hence, iPSC-derived podocytes offer an exciting alternative to culture podocyte-like cells from different donors over prolonged time. Here we report a simple and rapid one-step protocol that drives iPSC into podocyte-like cells in 10 days.

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Author information

Authors and Affiliations

  1. Division of Molecular and Computational Toxicology, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands

    Cormac Murphy, Paul Jennings & Anja Wilmes

  2. Division of Physiology, Medical University Innsbruck, Innsbruck, Austria

    Elisabeth Feifel & Gerhard Gstraunthaler

Authors
  1. Cormac Murphy
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  2. Elisabeth Feifel
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  3. Paul Jennings
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  4. Gerhard Gstraunthaler
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  5. Anja Wilmes
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Corresponding author

Correspondence to Anja Wilmes .

Editor information

Editors and Affiliations

  1. Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden

    Carl-Fredrik Mandenius

  2. Tissue Injury and Repair Group, University of Edinburgh, Edinburgh, UK

    James A. Ross

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Murphy, C., Feifel, E., Jennings, P., Gstraunthaler, G., Wilmes, A. (2019). A Protocol for One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes. In: Mandenius, CF., Ross, J. (eds) Cell-Based Assays Using iPSCs for Drug Development and Testing. Methods in Molecular Biology, vol 1994. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9477-9_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9477-9_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9476-2

  • Online ISBN: 978-1-4939-9477-9

  • eBook Packages: Springer Protocols

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