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Preparation of iPSCs for Targeted Proteomic Analysis

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Cell-Based Assays Using iPSCs for Drug Development and Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1994))

Abstract

Induced pluripotent stem cells have great potential as a human model system in regenerative medicine, disease modeling, and drug screening. However, extensive analysis of iPSC are required before their therapeutic applications. With recent developments in mass spectrometry and proteomics, this technique can become a great alternative to traditional genomic approaches for iPSC analysis. Here, we describe preparation of iPSC for targeted proteomic analysis, and measurement of pluripotency markers allowing for classification into either pluripotent or nonpluripotent cells.

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Acknowledgments

The authors declare no competing financial interests. The research leading to these results has received support from the Innovative Medicines Initiative Joint Undertaking under grant agreement no. 115439, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies’ in-kind contribution. All research at Great Ormond Street Hospital NHS Foundation Trust and UCL Great Ormond Street Institute of Child Health is made possible by the NIHR Great Ormond Street Hospital Biomedical Research Centre. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR the Department of Health, IMI JU, or EFPIA or the European Commission and are not liable for any use that may be made of the information contained therein. The authors would like to gratefully acknowledge the support of Leonard Wolfson and Peto foundations.

Author information

Authors and Affiliations

  1. Centre for Translational Omics, UCL Institute of Child Health, London, UK

    Anna Baud, Wendy E. Heywood & Kevin Mills

  2. MRC Laboratory for Molecular Cell Biology, University College London, London, UK

    Daniel Little & Paul Gissen

  3. Great Ormond Street Institute of Child Health, University College London, London, UK

    Paul Gissen

  4. NIHR Great Ormond Street Hospital Biomedical Research Centre, University College London, London, UK

    Paul Gissen

Authors
  1. Anna Baud
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  2. Wendy E. Heywood
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  3. Daniel Little
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  4. Paul Gissen
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  5. Kevin Mills
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Corresponding author

Correspondence to Anna Baud .

Editor information

Editors and Affiliations

  1. Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden

    Carl-Fredrik Mandenius

  2. Tissue Injury and Repair Group, University of Edinburgh, Edinburgh, UK

    James A. Ross

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Baud, A., Heywood, W.E., Little, D., Gissen, P., Mills, K. (2019). Preparation of iPSCs for Targeted Proteomic Analysis. In: Mandenius, CF., Ross, J. (eds) Cell-Based Assays Using iPSCs for Drug Development and Testing. Methods in Molecular Biology, vol 1994. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9477-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9477-9_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9476-2

  • Online ISBN: 978-1-4939-9477-9

  • eBook Packages: Springer Protocols

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