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Skin Tissue Engineering pp 91–103Cite as

Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1993))

Abstract

Adipose tissue-derived stromal cells (ASCs) are a promising candidates for cellular therapy in the field of regenerative medicine. ASCs are multipotent mesenchymal stem cell-like and reside in the stromal vascular fraction (SVF) of adipose tissue with the capacity to secrete a plethora of pro-regenerative growth factors. Future applications of ASCs may be restricted through (trans)national governmental policies that do not allow for use of nonhuman-derived (non-autologous) enzymes to isolate ASC. Besides, enzymatic isolation procedures are also time consuming. To overcome this issue, nonenzymatic isolation procedures to isolate ASCs or the SVF are being developed, such as the fractionation of adipose tissue procedure (FAT). This standardized procedure to isolate the stromal vascular fraction can be performed within 10–12 min. The short procedure time allows for intraoperative isolation of 1 mL of stromal vascular fraction derived from 10 mL of centrifuged adipose tissue. The stromal vascular fraction mostly contains blood vessels, extracellular matrix, and ASCs. However, based on the histological stainings an interdonor variation exists which might result in different therapeutic effects. The existing interdonor variations can be addressed by histological stainings and flow cytometry.

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Author information

Authors and Affiliations

  1. Plastic Surgery Department, Velthuis Kliniek, 3062 MB,, K.P. van der Mandelelaan 10, Rotterdam, The Netherlands

    Joris A. van Dongen & Hieronymus P. Stevens

  2. Department of Pathology and Medical Biology, University of Groningen and University Medical Centre of Groningen, Groningen, The Netherlands

    Joris A. van Dongen & Martin C. Harmsen

Authors
  1. Joris A. van Dongen
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  2. Martin C. Harmsen
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  3. Hieronymus P. Stevens
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Editor information

Editors and Affiliations

  1. University Children’s Hospital Zurich, Zurich, Switzerland

    Sophie Böttcher-Haberzeth  & Thomas Biedermann  & 

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van Dongen, J.A., Harmsen, M.C., Stevens, H.P. (2019). Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue. In: Böttcher-Haberzeth, S., Biedermann, T. (eds) Skin Tissue Engineering. Methods in Molecular Biology, vol 1993. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9473-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9473-1_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9472-4

  • Online ISBN: 978-1-4939-9473-1

  • eBook Packages: Springer Protocols

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